GCGR Polyklonaler Antikörper

GCGR Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 26784-1-AP

Synonyme

GCGR, GGR, GL R, glucagon receptor

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 26784-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26784-1-AP (GCGR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 26784-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26784-1-AP (GCGR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 26784-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26784-1-AP (GCGR antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 26784-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26784-1-AP (GCGR antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 26784-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26784-1-AP (GCGR antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 26784-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26784-1-AP (GCGR antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

26784-1-AP bindet in WB, IHC, IF, ELISA GCGR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GCGR fusion protein Ag24861
• Vollständiger Name: glucagon receptor
• Berechnetes Molekulargewicht: 477 aa, 54 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC104854
• Gene symbol: GCGR
• Gene ID (NCBI): 2642
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucagon receptor (GCGR) is a secretin-like (class B) family of G-protein coupled receptors (GPCRs). It plays an important role in elevating the glucose concentration in the blood and has thus become one of the promising therapeutic targets for the treatment of type 2 diabetes mellitus (PMID: 26236379). GCGR is a 62-kDa glycoprotein that contains at least four N-linked oligosaccharide chains (PMID: 2174441; 15245877). Defects in the gene of GCGR are a cause of non-insulin-dependent diabetes mellitus (NIDDM).

Publikationen

Species	Application	Title
mouse	WB,IF	iScience; Pro-α-cell-derived β-cells contribute to β-cell neogenesis induced by antagonistic glucagon receptor antibody in type 2 diabetic mice.; Authors - Xiaona Cui
mouse	WB	Mol Cell Endocrinol; Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.; Authors - Xinghong Guo
mouse	WB	Toxicology; Tributyltin exposure disturbs hepatic glucose metabolism in male mice.; Authors - Jing Xu
mouse	WB,IF	Am J Physiol Endocrinol Metab; Glucagon receptor blockage inhibits β-cell dedifferentiation through FoxO1; Authors - Kangli Wang
mouse	IHC,WB	Biomed Pharmacother; Si-Miao-Yong-An decoction preserves cardiac function and regulates GLC/AMPK/NF-κB and GLC/PPARα/PGC-1α pathways in diabetic mice; Authors - Lin Li