GCC1 Polyklonaler Antikörper

GCC1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (2)

Kat-Nr. : 16271-1-AP

Synonyme

GCC1, GCC1P, GCC88, Golgi coiled coil protein 1

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Galerie der Validierungsdaten

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  WB analysis using 16271-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16271-1-AP (GCC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 16271-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16271-1-AP (GCC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 16271-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16271-1-AP (GCC1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 16271-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16271-1-AP (GCC1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A431 using 16271-1-AP

  Immunofluorescent analysis of A431 cells using 16271-1-AP (GCC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maus-Eierstockgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

16271-1-AP bindet in WB, IHC, IF, ELISA GCC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GCC1 fusion protein Ag9339
• Vollständiger Name: GRIP and coiled-coil domain containing 1
• Berechnetes Molekulargewicht: 775 aa, 88 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC014100
• Gene symbol: GCC1
• Gene ID (NCBI): 79571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GCC1 (Golgi coiled coil protein 1), also known as GCC1P or GCC88, is a protein probably involved in maintaining Golgi structure. The MW of this protein is 105 kDa, and Catalog#16271-1-AP specially recognises the 105 kDa protein.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin.; Authors - Erpan Ahat
human	WB	Mol Biol Cell; Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.; Authors - Stephen C Ireland
human	WB	Mol Biol Cell; DjA1 maintains Golgi integrity via interaction with GRASP65.; Authors - Jie Li
human	WB	bioRxiv; SARS-CoV-2 triggers Golgi fragmentation via down-regulation of GRASP55 to facilitate viral trafficking.; Authors - Jianchao Zhang
human	WB,IF	Methods Mol Biol; Immunofluorescence Microscopy of the Mammalian Golgi Apparatus; Authors - Maryam Arab

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (04-28-2023)

clean band around the expected size

• Applications: Western Blot

FH

Jie (Verified Customer) (09-17-2020)

Works well for WB and IF with HeLa and CHO cells

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: HeLa, CHO