GBE1 Polyklonaler Antikörper

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• KD/KO Validated

GBE1 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 20313-1-AP

Synonyme

Brancher enzyme, GBE, GBE1, Glycogen branching enzyme

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Galerie der Validierungsdaten

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  WB analysis using 20313-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20313-1-AP (GBE1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 20313-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20313-1-AP (GBE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 20313-1-AP

  IP result of anti-GBE1(IP:20313-1-AP, 4ug; Detection:20313-1-AP 1:2000) with L02 cells lysate 1360 ug.

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  IF Staining of HeLa using 20313-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GBE1 antibody (20313-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, humanes Skelettmuskelgewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below

Produktinformation

20313-1-AP bindet in WB, IP, IF, ELISA GBE1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GBE1 fusion protein Ag14154
• Vollständiger Name: glucan (1,4-alpha-), branching enzyme 1
• Berechnetes Molekulargewicht: 702 aa, 80 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC012098
• Gene symbol: GBE1
• Gene ID (NCBI): 2632
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GBE1 (glucan (1,4-alpha-), branching enzyme 1) is essential for the globular and branched structure of glycogen, increasing solubility by creating a hydrophilic surface and reducing intracellular osmotic pressure. It may serve as a potential therapeutic target for treating LUAD(PMID: 31221150). The full sequence can be further processed into a mature form of 75 kDa.

Publikationen

Species	Application	Title
human	WB	Am J Hum Genet; Loss of C2orf69 defines a fatal autoinflammatory syndrome in humans and zebrafish that evokes a glycogen storage-associated mitochondriopathy.; Authors - Hui Hui Wong ; KD Validated
human	WB	Int J Biol Sci; PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer; Authors - Qian Ji
rat	WB	Carbohydr Polym; Liver fibrosis alters the molecular structures of hepatic glycogen; Authors - Yujun Wan
human	WB	iScience; MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.; Authors - Dong-Xue Ding
mouse	WB	J Proteome Res; Global Phosphoproteomic Analysis Reveals Significant Metabolic Reprogramming in the Termination of Liver Regeneration in Mice.; Authors - Jingzi Zhang
mouse	WB	Arch Biochem Biophys; Hallmarks of oxidative stress in the livers of aged mice with mild glycogen branching enzyme deficiency.; Authors - Dominika Malinska