GATA6 Polyklonaler Antikörper

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• KD/KO Validated

GATA6 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 55435-1-AP

Synonyme

GATA binding factor 6, GATA binding protein 6, GATA6, Transcription factor GATA 6

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 55435-1-AP

  WB result of GATA6 antibody (55435-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-GATA6 transfected MCF-7 cells.

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  WB analysis using 55435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55435-1-AP (GATA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 55435-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55435-1-AP (GATA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 55435-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 55435-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 55435-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 55435-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HuH-7 using 55435-1-AP

  1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human GATA6 (55435-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, BxPC-3-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HuH-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 1 publications below

Produktinformation

55435-1-AP bindet in WB, IHC, IF, FC, ELISA GATA6 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GATA binding protein 6
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60-64 kDa
• GenBank-Zugangsnummer: NM_005257
• Gene symbol: GATA6
• Gene ID (NCBI): 2627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GATA6 is thought to be important for regulating terminal differentiation and/or proliferation.

Publikationen

Species	Application	Title
mouse	WB	Environ Sci Technol; Perfluorinated Iodine Alkanes Promoted Neural Differentiation of mESCs by Targeting miRNA-34a-5p in Notch-Hes Signaling.; Authors - Zhihua Ren
mouse	WB,IF	iScience; Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency; Authors - Qian Ban
human	WB	FASEB J; Pulmonary expression of CYP2A13 and ABCB1 is regulated by FOXA2 and their genetic interaction is associated with lung cancer.; Authors - Chan Xiang
mouse	WB	Front Cell Dev Biol; ADNP Controls Gene Expression Through Local Chromatin Architecture by Association With BRG1 and CHD4.; Authors - XiaoYun Sun
human	WB	Cell Rep; METTL14 regulates chromatin bivalent domains in mouse embryonic stem cells; Authors - Mandi Mu
human	WB	Hum Cell; MicroRNA-10a-5p-mediated downregulation of GATA6 inhibits tumor progression in ovarian cancer; Authors - Feiying Gao