GATA4 Monoklonaler Antikörper

GATA4 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F5C6

Kat-Nr. : 68012-1-Ig

Synonyme

GATA binding factor 4, GATA binding protein 4, GATA4, Transcription factor GATA 4

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 68012-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 68012-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 68012-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 68012-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HEK-293-Zellen, HepG2-Zellen, NCCIT-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68012-1-Ig bindet in WB, ELISA GATA4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GATA4 fusion protein Ag30392
• Vollständiger Name: GATA binding protein 4
• Berechnetes Molekulargewicht: 442 aa, 45 kDa
• Beobachtetes Molekulargewicht: 44,45 kDa
• GenBank-Zugangsnummer: BC101580
• Gene symbol: GATA4
• Gene ID (NCBI): 2626
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GATA4 is a transcriptional activator. GATA4 binds to the consensus sequence 5'-AGATAG-3'. GATA4 acts as a transcriptional activator of ANF in cooperation with NKX2-5. Mutation of GATA4 will cause the atrial septal defect type 2 (ASD2).