GATA3 Monoklonaler Antikörper

GATA3 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

CloneNo.

1B11A8

Rezensionen (1)

Kat-Nr. : 66400-1-Ig

Synonyme

GATA binding factor 3, GATA binding protein 3, GATA3, HDR

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 66400-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66400-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66400-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66400-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66400-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66400-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66400-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66400-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 66400-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (66400-1-Ig, Clone: 1B11A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 66400-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66400-1-Ig (GATA3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66400-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed HepG2 cells using 66400-1-Ig(GATA3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 66400-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66400-1-Ig (GATA3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HeLa-Zellen, Jurkat-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen, HepG2-Zellen, humanes Mammakarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

66400-1-Ig bindet in WB, IHC, IF, CoIP, ELISA GATA3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GATA3 fusion protein Ag0174
• Vollständiger Name: GATA binding protein 3
• Berechnetes Molekulargewicht: 443 aa, 48 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC003070
• Gene symbol: GATA3
• Gene ID (NCBI): 2625
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression.

Publikationen

Species	Application	Title
human	WB,IHC	Drug Resist Updat; GATA3 mediates doxorubicin resistance by inhibiting CYB5R2-catalyzed iron reduction in breast cancer cells; Authors - Zhen Zhu
pig	WB	J Ethnopharmacol; Therapeutic effects and mechanisms study of Hanchuan Zupa Granule in a Guinea pig model of cough variant asthma.; Authors - Pengfei Li
mouse	WB	Pharm Biol; Modified BuShenYiQi formula alleviates experimental allergic asthma in mice by negative regulation of type 2 innate lymphoid cells and CD4 + type 9 helper T cells and the VIP-VPAC2 signalling pathway; Authors - Muhua Huang
mouse	WB	Int Arch Allergy Immunol; Lack of WDFY4 Aggravates Ovalbumin-Induced Asthma via Enhanced Th2 Cell Differentiation.; Authors - Yan Li
mouse	WB,IF	Evid Based Complement Alternat Med; Astragali Radix-Coptis Rhizoma Herb Pair Attenuates Atherosclerosis in ApoE-/- Mice by Regulating the M1/M2 and Th1/Th2 Immune Balance and Activating the STAT6 Signaling Pathway.; Authors - Zhaoyu Li
mouse	IHC	Chin J Integr Med; Acupoint Autohemotherapy Attenuates Atopic Dermatitis Lesions by Regulating Th1/Th2 Balance in DNCB-Induced BALB/c Mice.; Authors - Zhi-Wen Zeng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Renan (Verified Customer) (08-18-2022)

The fluorescence staining was noticeable, but the intensity was not so high, after using a tyramide signal amplification (TSA) protocol. The picture shows a lung lymphoid follicle, part of a 4-um FFPE tissue section. Heat-induced antigen retrieval was done with TRIS-EDTA buffer (pH 9.0) and GATA-3 was stained using a tyramide-conjugated Alexa Fluor 488 dye. CD3 is stained in yellow.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Lung lymphoid follicle

GATA3 Antibody Immunohistochemistry,Immunofluorescence validation (1:100 dilution) in Lung lymphoid follicle (Cat no:66400-1-Ig)