GATA2 Monoklonaler Antikörper

GATA2 Monoklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2B5A1

Kat-Nr. : 67722-1-Ig

Synonyme

FLJ45948, GATA binding protein 2, GATA2, NFE1B

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Galerie der Validierungsdaten

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  WB analysis using 67722-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of bEnd.3 using 67722-1-Ig

  bEnd.3 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67722-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67722-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67722-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67722-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67722-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67722-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67722-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 67722-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67722-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67722-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of L02 using 67722-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using GATA2 antibody (67722-1-Ig, Clone: 2B5A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of K-562 using 67722-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human GATA2 (67722-1-Ig, Clone:2B5A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of K-562 using 67722-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human GATA2 (67722-1-Ig, Clone:2B5A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, bEnd.3-Zellen, HEK-293-Zellen, HepG2-Zellen, HUVEC-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IF: L02-Zellen
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67722-1-Ig bindet in WB, IF, FC, ELISA GATA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GATA2 fusion protein Ag1557
• Vollständiger Name: GATA binding protein 2
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC018988
• Gene symbol: GATA2
• Gene ID (NCBI): 2624
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GATA2 is a member of GATA family of transcription factors that contains zinc fingers in DNA binding domain. GATA2 acts as a transcriptinal activator that regulates endothelin-1 expression in endothelial cells. Together with GATA3, GATA2 regulates adipocyte differentiation through molecular control of the preadpocyte-adipocyte transition. It also affect the activity of Rho inhibitor p190RhoGAP that controls capillary network formation in vitro and retinal angiogenesis in vivo.