GART Monoklonaler Antikörper

GART Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A11E2

Rezensionen (1)

Kat-Nr. : 67939-1-Ig

Synonyme

AIR synthase, AIRS, GAR transformylase, GARS, GART, GARTF, PAIS, PGFT, PRGS

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Galerie der Validierungsdaten

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  WB analysis using 67939-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67939-1-Ig (GART antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67939-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67939-1-Ig (GART antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 67939-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GART antibody (67939-1-Ig, Clone: 2A11E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67939-1-Ig bindet in WB, IF, ELISA GART und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GART fusion protein Ag30509
• Vollständiger Name: phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
• Berechnetes Molekulargewicht: 1010 aa, 108 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 108 kDa
• GenBank-Zugangsnummer: BC038958
• Gene symbol: GART
• Gene ID (NCBI): 2618
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (11-11-2022)

Great antibody. Very strong signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: HepG2 cell/ Mouse liver

GART Antibody Western Blot validation (1:8000 dilution) in HepG2 cell/ Mouse liver (Cat no:67939-1-Ig)