GALNT3 Polyklonaler Antikörper

GALNT3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 16716-1-AP

Synonyme

GalNAc T3, GALNT3, HFTC, HHS, pp GaNTase 3

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 16716-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16716-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 16716-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16716-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse liver using 16716-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of Transfected HEK-293 using 16716-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 16716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16716-1-AP (GALNT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16716-1-AP (GALNT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, DU 145-Zellen, HeLa-Zellen, Maushodengewebe, Mauslebergewebe, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

16716-1-AP bindet in WB, IHC, ELISA GALNT3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GALNT3 fusion protein Ag10140
• Vollständiger Name: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 3 (GalNAc-T3)
• Berechnetes Molekulargewicht: 633aa,73 kDa; 192aa,22 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC056246
• Gene symbol: GALNT3
• Gene ID (NCBI): 2591
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Cycle; LINC02535/miR-30a-5p/GALNT3 axis contributes to lung adenocarcinoma progression via the NF- κ B signaling pathway.; Authors - Yue Li
mouse	IHC	Bone; Growth-related skeletal changes and alterations in phosphate metabolism.; Authors - Toshimi Michigami
human	WB	J Exp Clin Cancer Res; LINC01296/miR-26a/GALNT3 axis contributes to colorectal cancer progression by regulating O-glycosylated MUC1 via PI3K/AKT pathway.; Authors - Bing Liu
human	IHC	Hum Cell; GalNAc-T3 and MUC1, a combined predictor of prognosis and recurrence in solitary pulmonary adenocarcinoma initially diagnosed as malignant solitary pulmonary nodule (≤ 3 cm).; Authors - Qiang Xie
rat	WB	Exp Ther Med; PMID: 29805509; Authors - Yanshao Liu
mouse	WB	Environ Toxicol; Cisplatin induces acute kidney injury by downregulating miR-30e-5p that targets Galnt3 to activate the AMPK signaling pathway; Authors - Weipu Mao