GALM Polyklonaler Antikörper

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• KD/KO Validated

GALM Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 16022-1-AP

Synonyme

Aldose 1 epimerase, BLOCK25, Galactose mutarotase, GALM, IBD1

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Galerie der Validierungsdaten

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  WB analysis using 16022-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16022-1-AP (GALM antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 16022-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16022-1-AP (GALM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16022-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16022-1-AP (GALM antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse spleen using 16022-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16022-1-AP (GALM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 16022-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16022-1-AP (GALM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 16022-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16022-1-AP (GALM antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16022-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16022-1-AP (GALM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16022-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16022-1-AP (GALM antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16022-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16022-1-AP (GALM antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Hirngewebe, humanes Milzgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

16022-1-AP bindet in WB, IHC, ELISA GALM und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GALM fusion protein Ag8842
• Vollständiger Name: galactose mutarotase (aldose 1-epimerase)
• Berechnetes Molekulargewicht: 342 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC019263
• Gene symbol: GALM
• Gene ID (NCBI): 130589
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GALM, also named as Aldose 1-epimerase, belongs to the aldose epimerase family. GALM is a mutarotase that catalyzes the interconversion of beta-D-galactose and alpha-D-galactose during galactose metabolism. GALM is involved in the maintenance of the equilibrium between the beta- and alpha- anomers of galactose, therefore ensuring a sufficient supply of the alpha-anomer for GALK1. Galm is also active on D-glucose, but its preference for galactose is higher than that of glucose. The calculated molecular weight of GALM is 38 kDa.

Publikationen

Species	Application	Title
human	WB	Genet Med; Biallelic GALM pathogenic variants cause a novel type of galactosemia.; Authors - Yoichi Wada
human	WB,IHC	Front Cell Dev Biol; Comprehensive Analyses of Glucose Metabolism in Glioma Reveal the Glioma-Promoting Effect of GALM.; Authors - Jiacheng Xu ; KD Validated