GABRG2 Polyklonaler Antikörper

GABRG2 Polyklonal Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 14104-1-AP

Synonyme

CAE2, ECA2, GABA A receptor gamma 2, GABAA receptor gamma 2, GABRG2, GEFSP3

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Galerie der Validierungsdaten

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  WB analysis using 14104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14104-1-AP (GABRG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14104-1-AP

  IP Result of anti-GABRG2 (IP:14104-1-AP, 4ug; Detection:14104-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IF Staining of mouse cerebellum using 14104-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using GABRG2 antibody (14104-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 14104-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug GABRG2 antibody (14104-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF: Maus-Cerebellum-Gewebe
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

14104-1-AP bindet in WB, IP, IHC, IF, FC, ELISA GABRG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GABRG2 fusion protein Ag5237
• Vollständiger Name: gamma-aminobutyric acid (GABA) A receptor, gamma 2
• Berechnetes Molekulargewicht: 467 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54-60 kDa
• GenBank-Zugangsnummer: BC059389
• Gene symbol: GABRG2
• Gene ID (NCBI): 2566
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GABRG2 (gamma-aminobutyric acid type A receptor subunit gamma2), which is expected to be located in cell membrane and expressed in brain and retina. The calculated molecular weight of the protein is 54 kDa. This antibody can recognize the 43 kDa band of target (PMID:12091434). And it also has glycosylation modification. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures.

Publikationen

Species	Application	Title
mouse	WB	Cell Death Dis; Transgenic overexpression of furin increases epileptic susceptibility.; Authors - Yi Yang
mouse	WB,IP	CNS Neurosci Ther; STK24 modulates excitatory synaptic transmission in epileptic hippocampal neurons.; Authors - Juan Yang
rat	IHC, IF	Neurosci Lett; Acupuncture Improves Locomotor Function by Enhancing GABA receptor Expression in Transient Focal Cerebral Ischemia Rats.; Authors - Qian Xu
mouse	WB,IF	Neuropharmacology; A role of GABAA receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine; Authors - Xiao-Hui Tang
mouse	WB	Front Endocrinol (Lausanne); Altered GnRH neuron and ovarian innervation characterize reproductive dysfunction linked to the Fragile X messenger ribonucleoprotein (Fmr1) gene mutation; Authors - Pedro A Villa
human	WB,IF	Nat Genet; Pan-cancer analysis identifies tumor-specific antigens derived from transposable elements; Authors - Nakul M Shah

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