G3BP1 Monoklonaler Antikörper
G3BP1 Monoklonal Antikörper für IF
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
Hausschwein, human, Maus, Ratte
Anwendung
IF
Konjugation
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E4A2
Kat-Nr. : CL488-66486
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in IF | sodium arsenite treated HeLa cells |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Immunfluoreszenz (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
CL488-66486 bindet in IF G3BP1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | G3BP1 fusion protein Ag3728 |
| Vollständiger Name | GTPase activating protein (SH3 domain) binding protein 1 |
| Berechnetes Molekulargewicht | 466 aa, 52 kDa |
| Beobachtetes Molekulargewicht | 68 kDa |
| GenBank-Zugangsnummer | BC006997 |
| Gene symbol | G3BP1 |
| Gene ID (NCBI) | 10146 |
| Konjugation | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Reinigungsmethode | Protein-G-Reinigung |
| Lagerungspuffer | BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3. |
| Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| IF protocol for CL Plus 488 G3BP1 antibody CL488-66486 | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tatyana (Verified Customer) (01-21-2023) | Excellent bright signal only after 30 min incubation (1:1000) at RT. PFA-fixed cells were used. Labels sodium arsenite induced stress granules well.
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