Foxp3 Monoklonaler Antikörper

Foxp3 Monoklonal Antikörper für FC, IHC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

Maus

Anwendung

IHC, IF, FC

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

3G3

Kat-Nr. : 65089-1-Ig

Synonyme

forkhead box P3, Foxp3, JM2, scurfin, sf

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Galerie der Validierungsdaten

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  IHC staining of mouse spleen using 65089-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 65089-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 65089-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of mouse splenocytes using 65089-1-Ig

  1X10^6 mouse splenocytes were intracellularly stained with 0.5 ug Anti-Mouse Foxp3 (65089-1-Ig, Clone:3G3) and FITC anti-Mouse IgG1 Antibody at dilution 1:50, or stained with 0.5 ug mouse IgG1 isotype control and FITC anti-Mouse IgG1 Antibody at dilution 1:50. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

65089-1-Ig bindet in IHC, IF, FC Foxp3 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: forkhead box P3
• GenBank-Zugangsnummer: BC132333
• Gene symbol: Foxp3
• Gene ID (NCBI): 20371
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Publikationen

Species	Application	Title
mouse	IHC	Thorac Cancer; Adiponectin-expressing Treg cells in experimental thymic tumor model; Authors - Yuki Hanamatsu
mouse	IF	Adv Sci (Weinh); Targeting L-Selectin Lymphocytes to Deliver Immunosuppressive Drug in Lymph Nodes for Durable Multiple Sclerosis Treatment; Authors - Yipeng Zhao
mouse	IF	Thorac Cancer; Adiponectin-expressing Treg-containing T cell fraction inhibits tumor growth in orthotopically implanted triple-negative breast cancer; Authors - Wakana Chikaishi

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011