Flotillin 2 Monoklonaler Antikörper

Flotillin 2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1E11G12

Rezensionen (1)

Kat-Nr. : 66881-1-Ig

Synonyme

ECS 1, ECS1, Epidermal surface antigen, ESA, ESA1, FLOT2, flotillin 2, M17S1

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Galerie der Validierungsdaten

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  WB analysis using 66881-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 66881-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66881-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66881-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66881-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66881-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Flotillin 2 antibody (66881-1-Ig, Clone: 1E11G12 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, A431-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Kaninchenhirngewebe, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

66881-1-Ig bindet in WB, IHC, IF, CoIP, ELISA Flotillin 2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Flotillin 2 fusion protein Ag28308
• Vollständiger Name: flotillin 2
• Berechnetes Molekulargewicht: 379 aa, 42 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC017292
• Gene symbol: FLOT2
• Gene ID (NCBI): 2319
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

Publikationen

Species	Application	Title
human	WB	J Dermatol Sci; MicroRNA-486-3p promotes the proliferation and metastasis of cutaneous squamous cell carcinoma by suppressing flotillin-2.; Authors - Xiangzhi Li
human	WB	Toxins (Basel); Interaction of Clostridium perfringens Epsilon Toxin with the Plasma Membrane: The Role of Amino Acids Y42, Y43 and H162; Authors - Skye Marshall
human	WB	Int J Oncol; Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2; Authors - Cheng Qian
human	WB,CoIP	Nat Commun; Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo; Authors - Jasjot Singh

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guorong (Verified Customer) (03-22-2022)

The band marked by the red dot may be the target

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: ARPE-19

Flotillin 2 Antibody Western Blot validation (1:2500 dilution) in ARPE-19 (Cat no:66881-1-Ig)