Flotillin 2 Polyklonaler Antikörper

Flotillin 2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 28208-1-AP

Synonyme

ECS 1, ECS1, Epidermal surface antigen, ESA, ESA1, FLOT2, flotillin 2, M17S1

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Galerie der Validierungsdaten

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  WB analysis using 28208-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28208-1-AP (Flotillin 2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 28208-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28208-1-AP (Flotillin 2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 28208-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Flotillin 2 antibody (28208-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

28208-1-AP bindet in WB, IHC, IF, ELISA Flotillin 2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Flotillin 2 fusion protein Ag28308
• Vollständiger Name: flotillin 2
• Berechnetes Molekulargewicht: 379 aa, 42 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC017292
• Gene symbol: FLOT2
• Gene ID (NCBI): 2319
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

Publikationen

Species	Application	Title
human	WB,IF	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
human	WB	J Exp Clin Cancer Res; RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization; Authors - Shuang Tao
human	WB	Int J Biol Sci; miR-34a-5p functions as a tumor suppressor in head and neck squamous cell cancer progression by targeting Flotillin-2.; Authors - Xiang Li
human	IHC	Int J Biol Sci; TBL1X and Flot2 form a positive feedback loop to promote metastasis in nasopharyngeal carcinoma.; Authors - Hongjuan Xu
human	WB	Oncol Rep; PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells.; Authors - Weidong Liu
human	WB	Alzheimers Dement; Amyloid futures in the expanding pathology of brain aging and dementia; Authors - Max A Thorwald

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guorong (Verified Customer) (03-22-2022)

Very weak bands with strong background were detected at 48 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: ARPE-19

Flotillin 2 Antibody Western Blot validation (1:1500 dilution) in ARPE-19 (Cat no:28208-1-AP)