Fibronectin Monoklonaler Antikörper

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• KD/KO Validated

Fibronectin Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen

1

CloneNo.

1G10F9

Kat-Nr. : CL488-66042

Synonyme

CIG, Cold insoluble globulin, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, ED B, Fibronectin, fibronectin 1, FINC, FN, FN1, FNZ, GFND, GFND2, LETS, MSF

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Galerie der Validierungsdaten

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  IF Staining of NIH/3T3 using CL488-66042

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CL488-66042 (Fibronectin antibody) at dilution of 1:100.

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  IF Staining of human colon cancer using CL488-66042

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 Fibronectin antibody (CL488-66042, Clone: 1G10F9 ) at dilution of 1:200.

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  IF Staining of human colon cancer using CL488-66042

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 Fibronectin antibody (CL488-66042, Clone: 1G10F9 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: NIH/3T3-Zellen, humanes Kolonkarzinomgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

CL488-66042 bindet in IHC, IF Fibronectin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fibronectin fusion protein Ag8016
• Vollständiger Name: fibronectin 1
• Berechnetes Molekulargewicht: 2386 aa, 263 kDa
• Beobachtetes Molekulargewicht: 263 kDa
• GenBank-Zugangsnummer: BC005858
• Gene symbol: FN1
• Gene ID (NCBI): 2335
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

Publikationen

Species	Application	Title
mouse	IHC	Front Pharmacol; Ginsenoside Rb1 Ameliorates Diabetic Arterial Stiffening via AMPK Pathway.; Authors - Xinyu Zhang