Frizzled 10 Polyklonaler Antikörper

Frizzled 10 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (2)

Kat-Nr. : 18175-1-AP

Synonyme

CD350, Frizzled 10, FZ 10, FZD10, FzE7, hFz10

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 18175-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18175-1-AP (Frizzled 10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18175-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18175-1-AP (Frizzled 10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18175-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18175-1-AP (Frizzled 10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 18175-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18175-1-AP (Frizzled 10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18175-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18175-1-AP (Frizzled 10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of human kidney using 18175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Frizzled 10 antibody (18175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 18175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Frizzled 10 antibody (18175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Nierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

18175-1-AP bindet in WB, IHC, IF, FC, ELISA Frizzled 10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Zebrafisch, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Frizzled 10 fusion protein Ag12867
• Vollständiger Name: frizzled homolog 10 (Drosophila)
• Berechnetes Molekulargewicht: 581 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC074998
• Gene symbol: FZD10
• Gene ID (NCBI): 11211
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FZD10 (Frizzled homolog 10) is a member of the frizzled gene family, and considered as a seven-transmembrane Wnt-signaling receptor. FZD10 is expressed at high levels on the cell surface of almost all synovial sarcoma tissues but absent in most normal organs except for placenta. Up-regulation of FZD10 mRNA in several types of human cells might lead to carcinogenesis through activation of the beta-catenin-TCF signaling pathway with some class of WNTs.

Publikationen

Species	Application	Title
mouse	WB,FC	Nat Commun; Progenitor translatome changes coordinated by Tsc1 increase perception of Wnt signals to end nephrogenesis.; Authors - Alison E Jarmas
chicken	IHC,IF	Development; Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.; Authors - Jianqiong Lin
zebrafish	WB	Development; Wnt/β-catenin signaling directly regulates Foxj1 expression and ciliogenesis in zebrafish Kupffer's vesicle.; Authors - Alissa Caron
human	WB	Oncotarget; Non coding RNA analysis in fibrolamellar hepatocellular carcinoma.; Authors - Benjamin A Farber
human	WB	Int J Oncol; Notch1 promotes hepatitis B virus X protein-induced hepatocarcinogenesis via Wnt/β-catenin pathway.; Authors - Qian Sun
goat	IHC	Cells; Integrative Analysis of Methylome and Transcriptome Reveals the Regulatory Mechanisms of Hair Follicle Morphogenesis in Cashmere Goat.; Authors - Shanhe Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Susanne (Verified Customer) (04-17-2023)

staining was not so nice like Frizzled 5, 7 or 9 from Proteintech

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Fibroblasts

Frizzled 10 Antibody Immunofluorescence validation (1:50 dilution) in Fibroblasts (Cat no:18175-1-AP)

FH

Sylvain (Verified Customer) (12-11-2018)

Perfect signal!

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: human colon cancer