FXYD6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FXYD6 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15805-1-AP

Synonyme

FXYD6, Phosphohippolin

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Galerie der Validierungsdaten

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  WB analysis using 15805-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15805-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15805-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 15805-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15805-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15805-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

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  FC experiment of SH-SY5Y using 15805-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug FXYD6 antibody (15805-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, humanes Hirngewebe, humanes Hodengewebe, Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Hodengewebe, humanes Lebergewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

15805-1-AP bindet in WB, IHC, IF, FC, ELISA FXYD6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FXYD6 fusion protein Ag8538
• Vollständiger Name: FXYD domain containing ion transport regulator 6
• Berechnetes Molekulargewicht: 95 aa, 11 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC018652
• Gene symbol: FXYD6
• Gene ID (NCBI): 53826
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The FXYD family is a group of small single-span transmembrane proteins characterized by a signature sequence containing an FXYD motif, two conserved glycines and a serine residue. Members of the FXYD family, including FXYD1 (phospholemman), FXYD2 (gamma subunit of Na,K-ATPase), FXYD3 (Mat8), FXYD4 (CHIF), FXYD5 (RIC), FXYD6 (phosphohippolin) and FXYD7, are tissue specific regulators of the Na,K-ATPase. FXYD6 is primarily expressed in the brain. It modulates the kinetic activity of Na,K-ATPase and has long-term physiological importance in maintaining cation homeostasis. It may play a role in endolymph composition and has a potential important role in neuronal excitability of the CNS during postnatal development and in the adult brain. On the SDS-PAGE FXYD6 migrates with an apparent molecular weight of approximately 20 kDa, which is larger than the calculated molecular weight of 10.5 kDa (PMID: 15193427; 17209044). The gene encodes FXYD6 is located on chromosome 11q23.3, and it might be a susceptibility gene of schizophrenia.

Publikationen

Species	Application	Title
mouse	IF	J Am Soc Nephrol; Single-Cell RNA Sequencing Reveals Renal Endothelium Heterogeneity and Metabolic Adaptation to Water Deprivation.; Authors - Sébastien J Dumas
human	WB,IF	Cancer Med; Identification of FXYD6 as the novel biomarker for glioma based on differential expression and DNA methylation; Authors - Weiliang Hou
human	WB	Biomed Res Int; FXYD6 Regulates Chemosensitivity by Mediating the Expression of Na+/K+-ATPase α1 and Affecting Cell Autophagy and Apoptosis in Colorectal Cancer.; Authors - Wen Luo ; KD Validated

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