FUT8 Monoklonaler Antikörper

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• KD/KO Validated

FUT8 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

CloneNo.

2F7H2

Kat-Nr. : 66118-1-Ig

Synonyme

Alpha (1,6) fucosyltransferase, alpha1 6FucT, Fucosyltransferase 8, FUT8

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 66118-1-Ig

  WB result of FUT8 antibody (66118-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FUT8 transfected MCF-7 cells.

• 

  WB analysis of MDA-MB-231 using 66118-1-Ig

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66118-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 66118-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 66118-1-Ig

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66118-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 40x lens.

• 

  IHC staining of human lung cancer using 66118-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 10x lens.

• 

  IHC staining of human breast cancer using 66118-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 10x lens.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-231-Zellen, Caco-2-Zellen, MCF-7-Zellen, MDA-MB-453s-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Produktinformation

66118-1-Ig bindet in WB, IHC, IF, ELISA FUT8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FUT8 fusion protein Ag18769
• Vollständiger Name: fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC025385
• Gene symbol: FUT8
• Gene ID (NCBI): 2530
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fucosyltransferase 8 (FUT8) is GDP-fucose-glycoprotein fucosyltransferase and is localized in Golgi apparatus. FUT8 is involved in protein glycosylation by catalyzing the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. FUT8 may be involved in human aging by regulating protein N-glycosylation and in the molecular pathogenesis of human pulmonary emphysema (PE).

Publikationen

Species	Application	Title
human	WB	Cancer Cell; A Systems Biology Approach Identifies FUT8 as a Driver of Melanoma Metastasis.; Authors - Praveen Agrawal ; KD Validated
human	WB	Am J Hum Genet; Biallelic Mutations in FUT8 Cause a Congenital Disorder of Glycosylation with Defective Fucosylation.; Authors - Bobby G Ng
human	IHC	Mol Cell Proteomics; Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei.; Authors - Lilli Saarinen
human	WB,IHC	Am J Cancer Res; Fucosyltransferase 8 modulates receptor tyrosine kinase activation and temozolomide resistance in glioblastoma cells.; Authors - Kuo-Chen Wei ; KD Validated
human	IHC	Mol Cell Proteomics; Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei; Authors - Lilli Saarinen
		Schizophr Res; Altered fucosyltransferase expression in the superior temporal gyrus of elderly patients with schizophrenia.; Authors - Toni M Mueller