FUT7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FUT7 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 18197-1-AP

Synonyme

Alpha (1,3) fucosyltransferase, Fuc TVII, Fucosyltransferase 7, Fucosyltransferase VII, FucT VII, FUT7, Selectin ligand synthase

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 18197-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18197-1-AP (FUT7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 18197-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18197-1-AP (FUT7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 18197-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18197-1-AP (FUT7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 18197-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18197-1-AP (FUT7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 18197-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18197-1-AP (FUT7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 18197-1-AP

  Immunofluorescent analysis of HeLa cells using 18197-1-AP (FUT7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

18197-1-AP bindet in WB, IHC, IF, ELISA FUT7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FUT7 fusion protein Ag12475
• Vollständiger Name: fucosyltransferase 7 (alpha (1,3) fucosyltransferase)
• Berechnetes Molekulargewicht: 342 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39-50 kDa
• GenBank-Zugangsnummer: BC074746
• Gene symbol: FUT7
• Gene ID (NCBI): 2529
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC, WB	J Inflamm Res; FUT7 Promotes the Epithelial-Mesenchymal Transition and Immune Infiltration in Bladder Urothelial Carcinoma.; Authors - Mulin Liu
human	WB,IHC	Exp Cell Res; FUT7 promotes the malignant transformation of follicular thyroid carcinoma through α1,3-Fucosylation of EGF receptor.; Authors - Huamin Qin ; KD Validated
human	WB	Evid Based Complement Alternat Med; Fut7 Promotes Adhesion and Invasion of Acute Lymphoblastic Leukemia Cells through the Integrin/Fak/Akt Pathway.; Authors - Fei He

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mark (Verified Customer) (02-05-2019)

The antibody was used at 1:1000 in standard western blot conditions to detect ectopically expressed human fut7 that was verified by qPCR. Antibody did not work.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human MDA-MB-231 cells overexpressing Fut7