FURIN Monoklonaler Antikörper

FURIN Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D1B9

Kat-Nr. : 67481-1-Ig

Synonyme

Dibasic processing enzyme, FUR, FURIN, PACE, PCSK3, SPC1

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Galerie der Validierungsdaten

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  WB analysis using 67481-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67481-1-Ig (FURIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 67481-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67481-1-Ig (FURIN antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67481-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67481-1-Ig (FURIN antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 67481-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67481-1-Ig (FURIN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 67481-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67481-1-Ig (FURIN antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, COLO 320-Zellen, HeLa-Zellen, JAR-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67481-1-Ig bindet in WB, IHC, ELISA FURIN und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FURIN fusion protein Ag13313
• Vollständiger Name: furin (paired basic amino acid cleaving enzyme)
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 90-110 kDa
• GenBank-Zugangsnummer: BC012181
• Gene symbol: FURIN
• Gene ID (NCBI): 5045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FURIN, also named as FUR, PACE, PCSK3, SPC1, Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. Glycosylation and other post-translational modifications can make the pre-pro furin run at 100-110 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa.