FTO Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FTO Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

103

Rezensionen (4)

Kat-Nr. : 27226-1-AP

Synonyme

FTO, KIAA1752

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Galerie der Validierungsdaten

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  WB analysis using 27226-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27226-1-AP (FTO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27226-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27226-1-AP (FTO antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 27226-1-AP

  IP result of anti-FTO(IP:27226-1-AP, 4ug; Detection:27226-1-AP 1:1000) with mouse brain tissue lysate 2080 ug.

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  IHC staining of mouse testis using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lymphoma using 27226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human lymphoma using 27226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver cancer using 27226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver cancer using 27226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 27226-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human FTO (27226-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Caco-2-Zellen, HEK-293T-Zellen, HEK-293-Zellen, HeLa-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Kolongewebe, humanes Leberkarzinomgewebe, humanes Magenkrebsgewebe, humanes Mammakarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Lymphomgewebe, humanes Leberkarzinomgewebe
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 13 publications below
• WB: See 96 publications below
• IHC: See 21 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 8 publications below

Produktinformation

27226-1-AP bindet in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA FTO und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FTO fusion protein Ag26095
• Vollständiger Name: fat mass and obesity associated
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: NM_001080432
• Gene symbol: FTO
• Gene ID (NCBI): 79068
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.

Publikationen

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
human	WB	Nat Genet; N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.; Authors - Yuan Li ; KO Validated
mouse	WB,RIP	Nat Cell Biol; The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.; Authors - Xiang-Qian Gao
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
human	WB	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen
human	WB	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Katherine (Verified Customer) (09-25-2023)

Quality was good.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

S (Verified Customer) (03-20-2023)

Very Good

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: AC16

FTO Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:27226-1-AP)

FH

SU (Verified Customer) (03-22-2022)

• Applications: Western Blot
• Cell Tissue Type: H9C2

FTO Antibody Western Blot validation ( dilution) in H9C2 (Cat no:27226-1-AP)

FH

P. (Verified Customer) (05-15-2021)

Excellent antibody!

• Applications: Western Blot
• Cell Tissue Type: H9C2

FTO Antibody Western Blot validation ( dilution) in H9C2 (Cat no:27226-1-AP)