Ferritin light chain Monoklonaler Antikörper

Ferritin light chain Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1F9F5

Kat-Nr. : 68068-1-Ig

Synonyme

Ferritin L subunit, Ferritin light chain, ferritin, light polypeptide, FTL

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Galerie der Validierungsdaten

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  WB analysis of A549 using 68068-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68068-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 68068-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 68068-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 68068-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FTL antibody (68068-1-Ig, Clone: 1F9F5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

68068-1-Ig bindet in WB, IHC, IF, ELISA Ferritin light chain und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Ferritin light chain fusion protein Ag20637
• Vollständiger Name: ferritin, light polypeptide
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC008439
• Gene symbol: FTL
• Gene ID (NCBI): 2512
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.

Publikationen

Species	Application	Title
human	WB	Front Oncol; Induction of Ferroptosis by Ophiopogonin-B Through Regulating the Gene Signature AURKA in NSCLC.; Authors - Liqiu Li
human	WB	Cell Death Dis; Hypoxia aggravates ferroptosis in RPE cells by promoting the Fenton reaction.; Authors - Yoshiyuki Henning
mouse	IHC	J Cardiovasc Dev Dis; Intraperitoneal Injection of Human Ferritin Heavy Chain Attenuates the Atherosclerotic Process in APOE-Knockout Mice; Authors - Wanzhong Yuan