FRK Polyklonaler Antikörper

FRK Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16197-1-AP

Synonyme

FRK, fyn related kinase, GTK, Protein tyrosine kinase 5, PTK5, RAK, Tyrosine protein kinase FRK

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Galerie der Validierungsdaten

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  WB analysis using 16197-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16197-1-AP (FRK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human urothelial carcinoma using 16197-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16197-1-AP (FRK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 16197-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16197-1-AP (FRK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16197-1-AP

  Immunofluorescent analysis of HepG2 cells, using FRK antibody 16197-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 16197-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug FRK antibody (16197-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16197-1-AP bindet in WB, IHC, IF, FC, ELISA FRK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FRK fusion protein Ag10372
• Vollständiger Name: fyn-related kinase
• Berechnetes Molekulargewicht: 505 aa, 56 kDa
• Beobachtetes Molekulargewicht: 54-57 kDa
• GenBank-Zugangsnummer: BC012916
• Gene symbol: FRK
• Gene ID (NCBI): 2444
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The fyn-related kinase (FRK) belongs to the tyrosine kinase family of protein kinases and is also named as PTK5(protein-tyrosine kinase 5), RAK(Nuclear tyrosine protein kinase). The Frk is a tyrosine kinase expressed in various tissues, such as the gastrointestinal tract and pancreatic islet cells. It affects pancreatic beta cell number during embryogenesis and promotes beta cell cytotoxic signals in response to streptozotocin(PMID:20157399). It may function as a tumor suppressor(PMID:19345329).