FOXN1 Monoklonaler Antikörper

FOXN1 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D8B12

Kat-Nr. : CL488-66337

Synonyme

FKHL20, forkhead box N1, Forkhead box protein N1, FOXN1, RONU, WHN

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Galerie der Validierungsdaten

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  FC experiment of A549 using CL488-66337

  1X10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human FOXN1 (CL488-66337, Clone:1D8B12) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: A549-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66337 bindet in FC (Intra) FOXN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FOXN1 fusion protein Ag18207
• Vollständiger Name: forkhead box N1
• Berechnetes Molekulargewicht: 648 aa, 69 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC146539
• Gene symbol: FOXN1
• Gene ID (NCBI): 8456
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXN1, also named as Forkhead box protein N1, is a 648 amino acid protein, which contains 1 fork-head DNA-binding domain and localizes in the nucleus. FOXN1 as transcriptional regulator regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. FOXN1 acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality.