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  • KD/KO Validated

FOLR1 Polyklonaler Antikörper

FOLR1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23355-1-AP

Synonyme

Adult folate binding protein, FBP, Folate receptor 1, folate receptor 1 (adult), Folate receptor alpha, Folate receptor, adult, FOLR, FOLR1, FR alpha, KB cells FBP, MOv18



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, JAR-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:12000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

23355-1-AP bindet in WB, IHC, IF, ELISA FOLR1 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen FOLR1 fusion protein Ag19959
Vollständiger Name folate receptor 1 (adult)
Berechnetes Molekulargewicht 257 aa, 30 kDa
Beobachtetes Molekulargewicht 38 kDa
GenBank-ZugangsnummerBC002947
Gene symbol FOLR1
Gene ID (NCBI) 2348
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Folate receptor 1 (FOLR1), also known as folate receptor alpha or adult folate-binding protein (FBP), is a 38-kDa glycoprotein belonging to the folate receptor family (PMID:15094207; 23528302). The receptor binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. FOLR1 expression is often limited to the apical surfaces of epithelium in the lung, kidney and choroid plexus but is differentially overexpressed in a variety of solid tumors such as ovarian cancer, non-small cell lung cancer, breast cancer, kidney cancer and high-grade osteosarcoma (PMID: 23528302; 23357463).

Protokolle

Produktspezifische Protokolle
WB protocol for FOLR1 antibody 23355-1-APProtokoll herunterladen
IHC protocol for FOLR1 antibody 23355-1-APProtokoll herunterladen
IF protocol for FOLR1 antibody 23355-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IHC

ACS Nano

Synchronization of Nanoparticle Sensitization and Radiosensitizing Chemotherapy through Cell Cycle Arrest Achieving Ultralow X-ray Dose Delivery to Pancreatic Tumors.

Authors - Divinah Manoharan
humanWB

J Am Chem Soc

Cancer Selective Target Degradation by Folate-Caged PROTACs.

Authors - Jing Liu
  • KD Validated
humanWB

ACS Appl Mater Interfaces

Design and Fabrication of Multifunctional Sericin Nanoparticles for Tumor Targeting and pH-Responsive Subcellular Delivery of Cancer Chemotherapy Drugs.

Authors - Lei Huang
humanWB

J Med Chem

Folate-Guided Protein Degradation by Immunomodulatory Imide Drug-Based Molecular Glues and Proteolysis Targeting Chimeras.

Authors - He Chen
  • KD Validated
humanWB

BMC Cancer

Cancer-associated fibroblasts induce sorafenib resistance of hepatocellular carcinoma cells through CXCL12/FOLR1

Authors - Jiali Zhao
humanWB

Nanotechnology

PMID: 28767041

Authors - Yuwei Hu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Marvin (Verified Customer) (12-01-2022)

The antibody had non-specific staining of the cytosol of MDA-MB-231 cells while performing immunofluorescent analysis.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Monolayer of MDA-MB-231 cells
FOLR1 Antibody Western Blot,Immunofluorescence validation (1:50 dilution) in Monolayer of MDA-MB-231 cells (Cat no:23355-1-AP)