FNTB Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

FNTB Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1F3B10

Kat-Nr. : CL594-66783

Synonyme

FNTB, FPTB, FTase beta

---

Galerie der Validierungsdaten

• 

  IF Staining of A431 using CL594-66783

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 FNTB antibody (CL594-66783, Clone: 1F3B10 ) at dilution of 1:400.

• 

  IF Staining of A431 using CL594-66783

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 FNTB antibody (CL594-66783, Clone: 1F3B10 ) at dilution of 1:400.

• 

  FC experiment of A431 using CL594-66783

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human FNTB (CL594-66783, Clone:1F3B10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: A431-Zellen
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66783 bindet in IF, FC (Intra) FNTB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FNTB fusion protein Ag27796
• Vollständiger Name: farnesyltransferase, CAAX box, beta
• Berechnetes Molekulargewicht: 437 aa, 49 kDa
• Beobachtetes Molekulargewicht: 44-45 kDa
• GenBank-Zugangsnummer: BC020232
• Gene symbol: FNTB
• Gene ID (NCBI): 2342
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.