FN3KRP Polyklonaler Antikörper

FN3KRP Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16312-1-AP

Synonyme

FN3K related protein, FN3K RP, FN3KL, FN3KRP, Ketosamine 3 kinase

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Galerie der Validierungsdaten

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  WB analysis using 16312-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16312-1-AP (FN3KRP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16312-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16312-1-AP (FN3KRP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16312-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16312-1-AP (FN3KRP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 16312-1-AP

  Immunofluorescent analysis of HeLa cells using 16312-1-AP (FN3KRP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16312-1-AP bindet in WB, IF,ELISA FN3KRP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FN3KRP fusion protein Ag9347
• Vollständiger Name: fructosamine 3 kinase related protein
• Berechnetes Molekulargewicht: 309 aa, 34 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC014408
• Gene symbol: FN3KRP
• Gene ID (NCBI): 79672
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fn3KRP (Fn3K-related protein) are believed to be the first examples of deglycating enzymes acting on the Amadori product to reverse the early stages of the Maillard reaction in vivo in higher organisms (PMID: 17337043).Fn3KRP possesses an ATP-binding domain that is structurally related to that of both the aminoglycoside kinases and eukaryotic protein kinases. Fn3KRP possesses an intrinsic activity towards ketosamine substrates (PMID: 18637789). Catalog#16312-1-AP is a rabbit polyclonal antibody raised against the full-length of human FN3KRP. The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein.