FMO3 Polyklonaler Antikörper

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• KD/KO Validated

FMO3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 17469-1-AP

Synonyme

Dimethylaniline oxidase 3, FMO 3, FMO form 2, FMO II, FMO3, FMOII, TMAU

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 17469-1-AP

  WB results of FMO3 antibody (17469-1-AP) with 8-week old CD1 male and female mice. (FMO3 is not expressed in male mice after 6-weeks of age and therefore are natural knockouts.).

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  WB analysis using 17469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17469-1-AP (FMO3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 17469-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17469-1-AP (FMO3 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 17469-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17469-1-AP (FMO3 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:100-1:500
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

17469-1-AP bindet in WB, IHC, ELISA FMO3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FMO3 fusion protein Ag11405
• Vollständiger Name: flavin containing monooxygenase 3
• Berechnetes Molekulargewicht: 532 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC032016
• Gene symbol: FMO3
• Gene ID (NCBI): 2328
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Microsomal flavin-containing monooxygenase (FMO) catalyzes the FAD-, NADPH-, and O2-dependent oxidation of a large number of xenobiotics containing soft nucleophiles,including alkaloids, pesticides, and pharmaceutical substances.Based on the cDNA sequence, the FMOs are classified into five subfamilies (FMO1 to FMO5)(PMID:11792679).In human beings ,FMO3 is predominant in the adult liver,but not appear sex-dependent in the tissue.In the mouse liver,its expression has been shown to be sex-dependent and expressed only in females(PMID:11996886).Defects in FMO3 are the cause of trimethylaminuria (TMAU).This antibody is specific to FMO3.

Publikationen

Species	Application	Title
mouse	WB	J Hazard Mater; Dietary titanium dioxide particles (E171) promote diet-induced atherosclerosis through reprogramming gut microbiota-mediated choline metabolism in APOE-/- mice.; Authors - Xiaoqiang Zhu
rat	WB	EBioMedicine; Akkermansia muciniphila prevents cold-related atrial fibrillation in rats by modulation of TMAO induced cardiac pyroptosis.; Authors - Yingchun Luo
mouse	WB	Front Microbiol; Berberine Improves Vascular Dysfunction by Inhibiting Trimethylamine-N-oxide via Regulating the Gut Microbiota in Angiotensin II-Induced Hypertensive Mice.; Authors - Zhichao Wang
rat	WB	Food Sci Biotechnol; Effects of black raspberry extract on gut microbiota, microbial metabolites, and expressions of the genes involved in cholesterol and bile acid metabolisms in rats fed excessive choline with a high-fat diet; Authors - Taehwan Lim