FLNA Monoklonaler Antikörper

FLNA Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G4H3

Kat-Nr. : CL488-67133

Synonyme

ABP 280, ABPX, Actin binding protein 280, Alpha filamin, DKFZp434P031, Filamin 1, Filamin A, FLN, FLN A, FLN1, FLNA, FMD, MNS, NHBP, Non muscle filamin, OPD, OPD1, OPD2

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Galerie der Validierungsdaten

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  IF Staining of HeLa using CL488-67133

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 FLNA antibody (CL488-67133, Clone: 1G4H3 ) at dilution of 1:200.

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  IF Staining of HeLa using CL488-67133

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488-conjugated FLNA antibody (CL488-67133, Clone: 1G4H3 ) at dilution of 1:400.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67133 bindet in IF FLNA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FLNA fusion protein Ag23291
• Vollständiger Name: filamin A, alpha (actin binding protein 280)
• Berechnetes Molekulargewicht: 2647 aa, 280 kDa
• Beobachtetes Molekulargewicht: 280 kDa
• GenBank-Zugangsnummer: BC014654
• Gene symbol: FLNA
• Gene ID (NCBI): 2316
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FLNA encodes a 280 kDa widely expressed actin binding protein called filamin A which can crosslinks actin filaments and links actin filaments to membrane glycoproteins to form a three-dimensional network (PMID: 29931263). And filamin A could interact with many other proteins, involved in receptor activation, cell migration and adhesion, cell proliferation, inflammation and tumorigenesis, studies have been reported that FLNA overexpressed in multiple types of tumors, suggesting FLNA may involved in cancer aggressiveness(PMID: 29100390). What's more, FLNA is an causative gene of periventricular nodular heterotopia (PNH) (PMID: 29062687)