FKBPL Monoklonaler Antikörper

FKBPL Monoklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

2H10B10

Kat-Nr. : 66389-1-Ig

Synonyme

DIR1, FK506 binding protein like, FKBPL, NG7, WISP39

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 66389-1-Ig

  Western blot analysis of FKBPL-specific in various cell lines using Proteintech antibody 66389-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66389-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66389-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66389-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66389-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66389-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66389-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 66389-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 66389-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66389-1-Ig (FKBPL antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, C6-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, Raji-Zellen, RAW 264.7-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66389-1-Ig bindet in WB, IHC, IF, ELISA FKBPL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FKBPL fusion protein Ag0112
• Vollständiger Name: FK506 binding protein like
• Berechnetes Molekulargewicht: 349 aa, 38 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC004168
• Gene symbol: FKBPL
• Gene ID (NCBI): 63943
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193) .It can be detected the band between 38 kDa and 48 kDa by western blot.

Publikationen

Species	Application	Title
human	WB	Cell Mol Life Sci; A placenta-on-a-chip model to determine the regulation of FKBPL and galectin-3 in preeclampsia; Authors - Sahar Masoumeh Ghorbanpour