FKBP52 Polyklonaler Antikörper

FKBP52 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22623-1-AP

Synonyme

51 kDa FK506 binding protein, 52 kDa FK506 binding protein, 52 kDa FKBP, 59 kDa immunophilin, FK506 binding protein 4, FK506 binding protein 4, 59kDa, FKBP 4, FKBP 52, FKBP4, FKBP51, FKBP52, FKBP59, HBI, HSP binding immunophilin, Hsp56, Immunophilin FKBP52, p52, p59, PPIase, PPIase FKBP4, Rotamase

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 22623-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22623-1-AP (FKBP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 22623-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22623-1-AP (FKBP52 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 22623-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22623-1-AP (FKBP52 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 22623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22623-1-AP (FKBP52 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 22623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22623-1-AP (FKBP52 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of PC-3 using 22623-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 22623-1-AP (FKBP52 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22623-1-AP bindet in WB, IHC, IF, ELISA FKBP52 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FKBP52 fusion protein Ag18704
• Vollständiger Name: FK506 binding protein 4, 59kDa
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC007924
• Gene symbol: FKBP4
• Gene ID (NCBI): 2288
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The large molecular-weight immunophilin, FKBP52, is a known target of the immunosuppressive drug FK506. FKBP52 exhibits peptidyl-prolyl cis-trans isomerase (PPIase) activity, which is inhibited by the binding of FK506-properties that it shares with the smaller but better-studied immunophilin, FKBP12(PMID:15381148). It is essential to AR-mediated induction of genes involved in sexually dimorphic development of the penis (NKX3-1), in erectile function of the mature organ (NOS2 and NOS3), and in promotion of apoptosis during development(PMID:20605780).