FKBP5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FKBP5 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 14155-1-AP

Synonyme

51 kDa FK506 binding protein, 51 kDa FKBP, AIG6, Androgen regulated protein 6, FF1 antigen, FK506 binding protein 5, FKBP 5, FKBP 51, FKBP5, FKBP51, FKBP54, HSP90 binding immunophilin, P54, PPIase, PPIase FKBP5, Ptg 10, Rotamase

---

Galerie der Validierungsdaten

• 

  WB analysis of K-562 using 14155-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14155-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14155-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14155-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14155-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 14155-1-AP

  IP Result of anti-FKBP5 (IP:14155-1-AP, 3ug; Detection:14155-1-AP 1:1000) with K-562 cells lysate 1500ug.

• 

  IHC staining of human colon cancer using 14155-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14155-1-AP (FKBP5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 14155-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14155-1-AP (FKBP5 antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, Maus-Thymusgewebe
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

14155-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA FKBP5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FKBP5 fusion protein Ag5337
• Vollständiger Name: FK506 binding protein 5
• Berechnetes Molekulargewicht: 457 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC042605
• Gene symbol: FKBP5
• Gene ID (NCBI): 2289
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FKBP5(Peptidyl-prolyl cis-trans isomerase FKBP5 ) interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Unlike the predominant T-cell expression of the mouse protein, FKBP5 is expressed as a 51-kD protein in a variety of tissues but not in brain, colon, or lung(PMID: 9125197).

Publikationen

Species	Application	Title
human	IF	Cell Stem Cell; Decoding the temporal and regional specification of microglia in the developing human brain.; Authors - Yanxin Li
human	WB	EMBO J; USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.; Authors - Kuntian Luo ; KD Validated
mouse	IHC	Nutrients; Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus; Authors - Tova M Bergsten
mouse	IHC	Mol Cancer Ther; Cell intrinsic role of COX-2 in pancreatic cancer development.; Authors - Hill Reginald R
mouse	WB	RNA; The co-chaperones Fkbp4/5 control Argonaute2 expression and facilitate RISC assembly.; Authors - Martinez Natalia J NJ
mouse	WB	Biochem Pharmacol; Developmental nicotine exposure elicits multigenerational disequilibria in proBDNF proteolysis and glucocorticoid signaling in the frontal cortices, striata, and hippocampi of adolescent mice.; Authors - Jordan M Buck