FKBP14 Polyklonaler Antikörper

FKBP14 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15884-1-AP

Synonyme

22 kDa FK506 binding protein, 22 kDa FKBP, FK506 binding protein 14, FKBP 14, FKBP 22, FKBP14, FKBP22, PPIase FKBP14, Rotamase

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 15884-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15884-1-AP (FKBP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15884-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15884-1-AP (FKBP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15884-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15884-1-AP (FKBP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Hirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 3 publications below

Produktinformation

15884-1-AP bindet in WB, IF, ELISA FKBP14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FKBP14 fusion protein Ag8668
• Vollständiger Name: FK506 binding protein 14, 22 kDa
• Berechnetes Molekulargewicht: 211 aa, 24 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC005206
• Gene symbol: FKBP14
• Gene ID (NCBI): 55033
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FKBP14, also named as FKBP22, belongs to the family of FK506-binding peptidyl-prolyl cis-trans isomerases (PPIases). PPIases accelerate the folding of proteins during protein synthesis. FKBP14 is localized in the endoplasmic reticulum (ER) and that deficiency of FKBP14 leads to enlarged ER cisterns in dermal fibroblasts in vivo. FKBP14 is about 22kd.

Publikationen

Species	Application	Title
human	WB,IF	Am J Hum Genet; Mutations in FKBP14 cause a variant of Ehlers-Danlos syndrome with progressive kyphoscoliosis, myopathy, and hearing loss.; Authors - Baumann Matthias M
human	WB,IF	Sci Rep; The novel missense mutation Met48Lys in FKBP22 changes its structure and functions.; Authors - Yoshihiro Ishikawa
human	IF	Hum Mutat; Kyphoscoliotic Ehlers-Danlos syndrome caused by pathogenic variants in FKBP14: Further insights into the phenotypic spectrum and pathogenic mechanisms; Authors - Birute Burnyte
human	WB	Int J Mol Sci; Local Net Charge State of Collagen Triple Helix Is a Determinant of FKBP22 Binding to Collagen III; Authors - Yoshihiro Ishikawa