FIS1 Monoklonaler Antikörper

FIS1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

CloneNo.

2F10E1

Rezensionen (1)

Kat-Nr. : 66635-1-Ig

Synonyme

CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11

---

Galerie der Validierungsdaten

• 

  WB analysis using 66635-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66635-1-Ig (FIS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66635-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66635-1-Ig (FIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66635-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66635-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66635-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66635-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66635-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66635-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66635-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66635-1-Ig (FIS1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Ovarialkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

66635-1-Ig bindet in WB, IHC, IF, ELISA FIS1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Fisch, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FIS1 fusion protein Ag1409
• Vollständiger Name: fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC009428
• Gene symbol: FIS1
• Gene ID (NCBI): 51024
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (21257639, 21459773, 23333625)

Publikationen

Species	Application	Title
human	WB	J Pineal Res; Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics; Authors - Ye Young Shin
mouse	WB	Front Immunol; Metformin Modulates T Cell Function and Alleviates Liver Injury Through Bioenergetic Regulation in Viral Hepatitis.; Authors - Lanman Xu
fish	WB	J Physiol Sci; Cardioprotective responses to aerobic exercise-induced physiological hypertrophy in zebrafish heart; Authors - Zhanglin Chen
human	IF	Biol Pharm Bull; Ampelopsin inhibits breast cancer cell growth through mitochondrial apoptosis pathway; Authors - YUE LI
rat	IHC	J Mol Histol; Roles of HIF-1α/BNIP3 mediated mitophagy in mitochondrial dysfunction of letrozole-induced PCOS rats; Authors - Fan Wang
rat	WB	Aging (Albany NY); Jian-Pi-Yi-Shen decoction inhibits mitochondria-dependent granulosa cell apoptosis in a rat model of POF; Authors - Xiao-Lin Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Chun (Verified Customer) (06-19-2022)

This antibody worked very well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HeLa cells and HEK293 cells