FIBP Polyklonaler Antikörper

FIBP Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15968-1-AP

Synonyme

FGFIBP, FIBP, FIBP 1

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Galerie der Validierungsdaten

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  WB analysis of BxPC-3 using 15968-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15968-1-AP (FIBP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 15968-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15968-1-AP (FIBP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of mouse pancreas using 15968-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15968-1-AP (FIBP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of BxPC-3 using 15968-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15968-1-AP (FIBP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15968-1-AP (FIBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 15968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15968-1-AP (FIBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15968-1-AP

  Immunofluorescent analysis of HepG2 cells using 15968-1-AP (FIBP antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15968-1-AP bindet in WB, IHC, IF, ELISA FIBP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FIBP fusion protein Ag8743
• Vollständiger Name: fibroblast growth factor (acidic) intracellular binding protein
• Berechnetes Molekulargewicht: 357 aa, 41 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC014388
• Gene symbol: FIBP
• Gene ID (NCBI): 9158
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FIBP, also named as Acidic fibroblast growth factor intracellular binding protein, is a 364 amino acid protein, which is highly expressed in heart, skeletal muscle and pancreas. FIBP may be involved in mitogenic function of FGF1.