FHL2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FHL2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 21619-1-AP

Synonyme

AAG11, DRAL, FHL 2, FHL2, four and a half LIM domains 2, LIM domain protein DRAL, Skeletal muscle LIM protein 3, SLIM 3, SLIM3

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Galerie der Validierungsdaten

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  WB analysis of mouse ovary using 21619-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21619-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 21619-1-AP

  IP Result of anti-FHL2 (IP:21619-1-AP, 4ug; Detection:21619-1-AP 1:1000) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of human heart using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21619-1-AP (FHL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21619-1-AP (FHL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 21619-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 21619-1-AP (FHL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 21619-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FHL2 antibody (21619-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, hTERT-RPE1-Zellen, NIH/3T3-Zellen, U2OS-Zellen
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 4 publications below

Produktinformation

21619-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA FHL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FHL2 fusion protein Ag1378
• Vollständiger Name: four and a half LIM domains 2
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC014397
• Gene symbol: FHL2
• Gene ID (NCBI): 2274
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Commun; A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.; Authors - Xiaojie Xu
human	WB,IF,CoIP	EBioMedicine; Up-regulated FHL2 inhibits ovulation through interacting with androgen receptor and ERK1/2 in polycystic ovary syndrome.; Authors - Ruiqiong Zhou
human,mouse	WB,IHC,IF,CoIP	Cell Death Dis; FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells; Authors - Chen Wang ; KD Validated
mouse	CoIP,WB	iScience; Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice; Authors - Lu Ren
human	WB	Int J Mol Sci; FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3; Authors - Zhiqiang Xu ; KD Validated
human	WB, IHC	J Virol; Structural and Functional Characterization of Host FHL1 Protein Interaction with Hypervariable Domain of Chikungunya Virus nsP3 Protein.; Authors - Tetyana Lukash