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  • KD/KO Validated

FGFR3 Monoklonaler Antikörper

FGFR3 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F3G1

Kat-Nr. : 66954-1-Ig

Synonyme

ACH, CD333, CEK2, FGFR 3, FGFR3, HSFGFR3EX, JTK4



Geprüfte Anwendungen

Erfolgreiche Detektion in WBLNCaP-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, L02-Zellen, L-929-Zellen, NCI-H1299-Zellen
Erfolgreiche Detektion in IHCMaushodengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:200-1:800
Immunfluoreszenz (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66954-1-Ig bindet in WB, IHC, IF, ELISA FGFR3 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen FGFR3 fusion protein Ag26290
Vollständiger Name fibroblast growth factor receptor 3
Berechnetes Molekulargewicht 87 kDa
Beobachtetes Molekulargewicht 125-135 kDa
GenBank-ZugangsnummerNM_000142
Gene symbol FGFR3
Gene ID (NCBI) 2261
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibroblast growth factors (FGFs) are polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing (PMID: 1847508). The human FGF receptor family, a subfamily of receptor tyrosine kinases (RTKs), comprises of four family members-FGFR1, FGFR2, FGFR3 and FGFR4 (PMID: 23900974). Each receptor contains an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. FGFR3 binds acidic and basic fibroblast GH and plays a role in bone development and maintenance. Mutations in the FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Due to frequent mutations in certain cancers, FGFR3 gene has also been associated with tumor progression.

Protokolle

Produktspezifische Protokolle
WB protocol for FGFR3 antibody 66954-1-IgProtokoll herunterladen
IHC protocol for FGFR3 antibody 66954-1-IgProtokoll herunterladen
IF protocol for FGFR3 antibody 66954-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Ann Rheum Dis

FGFR3 deficiency enhances CXCL12-dependent chemotaxis of macrophages via upregulating CXCR7 and aggravates joint destruction in mice.

Authors - Liang Kuang
  • KD Validated
humanWB

Cells

Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.

Authors - Damian Kołat
mouseWB

Bioengineered

The effects of Omarigliptin on promoting osteoblastic differentiation.

Authors - Fake Liao
humanWB

J Exp Clin Cancer Res

HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway.

Authors - Kai Guo
humanWB

J Exp Clin Cancer Res

Dysregulation and prometastatic function of glycosyltransferase C1GALT1 modulated by cHP1BP3/ miR-1-3p axis in bladder cancer.

Authors - Zengqi Tan
humanIF

Tissue Eng Part A

The UC-MSCbFGF/scaffold system accelerates the healing of the uterine full-thickness injury

Authors - Limin Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Clarisse (Verified Customer) (12-12-2022)

This antibody works very well on tissue fixed overnight at 4C with 2% PFA. No antigen retrieval required.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:750
  • Cell Tissue Type: Human cerebral organoids