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FGFR3 Monoklonaler Antikörper
FGFR3 Monoklonal Antikörper für IF, IHC, WB, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human, Maus
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
CloneNo.
1F3G1
Kat-Nr. : 66954-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | LNCaP-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, L02-Zellen, L-929-Zellen, NCI-H1299-Zellen |
Erfolgreiche Detektion in IHC | Maushodengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF | HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunhistochemie (IHC) | IHC : 1:200-1:800 |
Immunfluoreszenz (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 2 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
Produktinformation
66954-1-Ig bindet in WB, IHC, IF, ELISA FGFR3 und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | FGFR3 fusion protein Ag26290 |
Vollständiger Name | fibroblast growth factor receptor 3 |
Berechnetes Molekulargewicht | 87 kDa |
Beobachtetes Molekulargewicht | 125-135 kDa |
GenBank-Zugangsnummer | NM_000142 |
Gene symbol | FGFR3 |
Gene ID (NCBI) | 2261 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-G-Reinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Fibroblast growth factors (FGFs) are polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing (PMID: 1847508). The human FGF receptor family, a subfamily of receptor tyrosine kinases (RTKs), comprises of four family members-FGFR1, FGFR2, FGFR3 and FGFR4 (PMID: 23900974). Each receptor contains an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. FGFR3 binds acidic and basic fibroblast GH and plays a role in bone development and maintenance. Mutations in the FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Due to frequent mutations in certain cancers, FGFR3 gene has also been associated with tumor progression.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for FGFR3 antibody 66954-1-Ig | Protokoll herunterladen |
IHC protocol for FGFR3 antibody 66954-1-Ig | Protokoll herunterladen |
IF protocol for FGFR3 antibody 66954-1-Ig | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Ann Rheum Dis FGFR3 deficiency enhances CXCL12-dependent chemotaxis of macrophages via upregulating CXCR7 and aggravates joint destruction in mice.
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Cells Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer. | ||
J Exp Clin Cancer Res HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway. | ||
J Exp Clin Cancer Res Dysregulation and prometastatic function of glycosyltransferase C1GALT1 modulated by cHP1BP3/ miR-1-3p axis in bladder cancer. | ||
Tissue Eng Part A The UC-MSCbFGF/scaffold system accelerates the healing of the uterine full-thickness injury |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Clarisse (Verified Customer) (12-12-2022) | This antibody works very well on tissue fixed overnight at 4C with 2% PFA. No antigen retrieval required.
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