FGF8 Polyklonaler Antikörper

FGF8 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 20711-1-AP

Synonyme

AIGF, Androgen induced growth factor, FGF 8, FGF8, Fibroblast growth factor 8, HBGF 8, KAL6

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Galerie der Validierungsdaten

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  WB analysis using 20711-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20711-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20711-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20711-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HeLa-Zellen, L02-Zellen, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

20711-1-AP bindet in WB, IHC, IF, ELISA FGF8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: fibroblast growth factor 8 (androgen-induced)
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: NM_006119
• Gene symbol: FGF8
• Gene ID (NCBI): 2253
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FGF8, also named as AIGF and HBGF-8, belongs to the heparin-binding growth factors family. FGF8 stimulates growth of the cells in an autocrine manner. It mediates hormonal action on the growth of cancer cells. Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6). Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH). The antibody is specific to FGF8.

Publikationen

Species	Application	Title
human	WB,IHC	Carcinogenesis; The oncoprotein HBXIP enhances angiogenesis and growth of breast cancer through modulating FGF8 and VEGF.; Authors - Fabao Liu
human	IF	Oncoimmunology; Immunoreactivity against fibroblast growth factor 8 in alveolar rhabdomyosarcoma patients and its involvement in tumor aggressiveness.; Authors - Elena Poli
human	IHC	Oncol Lett; Systematic analysis of expression profiles and prognostic significance of the FGF gene family in pancreatic adenocarcinoma; Authors - Yu-Xin Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Paolo (Verified Customer) (12-12-2019)

It works fine in wb, in IF differences in FGF8 intracellular localization were observed according to the fixative solution used

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1/500