FDPS Monoklonaler Antikörper

FDPS Monoklonal Antikörper für FC (Intra), WB

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B8A7

Kat-Nr. : CL488-67972

Synonyme

FDPS, FPP synthase, FPPS, FPS, KIAA1293

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Galerie der Validierungsdaten

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  WB analysis using CL488-67972

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-67972 (FDPS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using CL488-67972

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FDPS (CL488-67972, Clone:3B8A7) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67972 bindet in WB, FC (Intra) FDPS und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FDPS fusion protein Ag9247
• Vollständiger Name: farnesyl diphosphate synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase)
• Berechnetes Molekulargewicht: 419 aa, 48 kDa
• Beobachtetes Molekulargewicht: 38-39 kDa
• GenBank-Zugangsnummer: BC010004
• Gene symbol: FDPS
• Gene ID (NCBI): 2224
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FPPS (also known as FDPS) is a key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. The expression of FPPS was elevated in prostate tumor cell lines, indicating its potential role in prostate cancer progression.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C