FCGR2A / CD32a Monoklonaler Antikörper

FCGR2A / CD32a Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D3F1

Kat-Nr. : CL488-66529

Synonyme

CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A / CD32a, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a

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Galerie der Validierungsdaten

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  IF Staining of THP-1 using CL488-66529

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 488 FCGR2A / CD32a antibody (CL488-66529, Clone: 1D3F1 ) at dilution of 1:50.

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  IF Staining of human tonsillitis using CL488-66529

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66529 (FCGR2A / CD32a antibody) at dilution of 1:50.

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  IF Staining of human tonsillitis using CL488-66529

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66529 (FCGR2A / CD32a antibody) at dilution of 1:50.

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  FC experiment of THP-1 using CL488-66529

  1X10^6 THP-1 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human FCGR2A / CD32a (CL488-66529, Clone:1D3F1) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: THP-1-Zellen, humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: THP-1-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66529 bindet in IF, FC (Intra) FCGR2A / CD32a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FCGR2A / CD32a fusion protein Ag25878
• Vollständiger Name: Fc fragment of IgG, low affinity IIa, receptor (CD32)
• Berechnetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC020823
• Gene symbol: FCGR2A
• Gene ID (NCBI): 2212
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.