FBXO32 Monoklonaler Antikörper

FBXO32 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

23

CloneNo.

3C2G3

Kat-Nr. : 67172-1-Ig

Synonyme

Atrogin 1, F box only protein 32, F box protein 32, Fbx32, FBXO32, FLJ32424, MAFbx, Muscle atrophy F box protein

---

Galerie der Validierungsdaten

• 

  WB analysis using 67172-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 67172-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 67172-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig skeletal muscle using 67172-1-Ig

  pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 67172-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 67172-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 67172-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 67172-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 67172-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBXO32 antibody (67172-1-Ig, Clone: 3C2G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 67172-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBXO32 antibody (67172-1-Ig, Clone: 3C2G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Hausschwein-Herzgewebe, Hausschwein-Skelettmuskelgewebe, humanes Herzgewebe, Kaninchen-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:10000-1:50000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 16 publications below
• IHC: See 10 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

67172-1-Ig bindet in WB, IP, IHC, IF, ELISA FBXO32 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FBXO32 fusion protein Ag25247
• Vollständiger Name: F-box protein 32
• Berechnetes Molekulargewicht: 355 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC024030
• Gene symbol: FBXO32
• Gene ID (NCBI): 114907
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.

Publikationen

Species	Application	Title
mouse	WB	Cell Biol Toxicol; Statins induce skeletal muscle atrophy via GGPP depletion-dependent myostatin overexpression in skeletal muscle and brown adipose tissue.; Authors - Lai Wang
pig	WB	J Anim Sci Biotechnol; Protective effects of taurine against muscle damage induced by diquat in 35 days weaned piglets.; Authors - Chaoyue Wen
canine	WB,IHC	Life Sci; N-acetylcysteine alleviates oxidative stress and apoptosis and prevents skeletal muscle atrophy in type 1 diabetes mellitus through the NRF2/HO-1 pathway; Authors - Qingyu Ding
mouse	WB	J Ethnopharmacol; Saponin extract from Achyranthes bidentata Blume alleviates disuse-induced muscle atrophy through PI3K/Akt signaling pathway; Authors - Yi Shi
mouse	WB	Front Cell Dev Biol; Phosphorylation of Dynamin-Related Protein 1 (DRP1) Regulates Mitochondrial Dynamics and Skeletal Muscle Wasting in Cancer Cachexia.; Authors - Xiangyu Mao
mouse	WB	Exp Cell Res; Resistance exercise alleviates dexamethasone-induced muscle atrophy via Sestrin2/MSTN pathway in C57BL/6J mice; Authors - Yang Yang