• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

FBF1 Polyklonaler Antikörper

FBF1 Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-11531

Synonyme

Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, FLJ00103, FLJ13996, HCG1989313, isoform CRA_b, KIAA1863

Galerie der Validierungsdaten

Filter:
  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated FBF1 Polyclonal anti (CL488-11531)

    IF Staining of A549 using CL488-11531

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 FBF1 antibody (CL488-11531) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CoraLite® Plus 488-conjugated FBF1 Polyclonal anti (CL488-11531)

    IF Staining of HUVEC using CL488-11531

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 FBF1 antibody (CL488-11531) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IFA549-Zellen, HUVEC-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-11531 bindet in IF FBF1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen FBF1 fusion protein Ag2106
Vollständiger Name Fas (TNFRSF6) binding factor 1
Berechnetes Molekulargewicht1133 aa, 125 kDa
Beobachtetes Molekulargewicht 130 kDa
GenBank-ZugangsnummerBC023549
Gene symbol FBF1
Gene ID (NCBI) 85302
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 FBF1 antibody CL488-11531Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Karine (Verified Customer) (02-26-2024)

FBF1-Coralite488 (in green) does label one extremity of the primary cilium labeled with ARL13B (in red) in RPE1 cells whose nuclei appear in purple and cell contour in grey with Brightfield. It is quite specific when used at 1/1000 overnight at 4°C. There is a bit more aspecific signal than with the uncoupled antibdy but it remains quite reasonable.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: RPE1 cells
CL Plus 488 FBF1 Antibody Immunofluorescence validation (1/1000 dilution) in RPE1 cells (Cat no:CL488-11531)