FASTKD3 Polyklonaler Antikörper

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• KD/KO Validated

FASTKD3 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 18392-1-AP

Synonyme

FAST kinase domains 3, FASTKD3

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 18392-1-AP

  WB result of FASTKD3 antibody (18392-1-AP, 1:200) with HeLa and 293 cell lysate by Maciej Szewczyk University of Warsaw.

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  WB analysis of HepG2 using 18392-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18392-1-AP (FASTKD3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 18392-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18392-1-AP (FASTKD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 18392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18392-1-AP (FASTKD3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18392-1-AP (FASTKD3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of transfected cells using 18392-1-AP

  IF result of anti-FASTKD3 (18392-1-AP, 1:200) with transfected COS7 cell by Dr. Nancy Kedersha.

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  IF Staining of HepG2 using 18392-1-AP

  Immunofluorescent analysis of HepG2 cells using 18392-1-AP (FASTKD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 18392-1-AP

  Immunofluorescent analysis of HeLa cells using 18392-1-AP (FASTKD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Transfizierte Zellen, HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

18392-1-AP bindet in WB, IHC, IF,ELISA FASTKD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FASTKD3 fusion protein Ag13083
• Vollständiger Name: FAST kinase domains 3
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC001295
• Gene symbol: FASTKD3
• Gene ID (NCBI): 79072
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAST kinase domain-containing protein 3(FASTKD3) belongs to FASTKD family.FASTKD3 is an essential component of mitochondrial respiration that may modulate energy balance in cells exposed to adverse conditions by functionally coupling mitochondrial protein synthesis to respiration. The expression of endogenous FASTKD3 is very weak. This antibody detects the c-terminal of FASTKD3.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; Role of FAST kinase domains 3 (FASTKD3) in post-transcriptional regulation of mitochondrial gene expression.; Authors - Erik Boehm ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Erik (Verified Customer) (02-25-2021)

We used the antibody at 1:500 dilution, and it produced several bands, including the expected one at 75kda approx (as per UniProt annotation). The unexpected bands were, however, more intense than the expected one.

• Applications: Western Blot
• Cell Tissue Type: MIAPACA2 cell line

FASTKD3 Antibody Western Blot validation ( dilution) in MIAPACA2 cell line (Cat no:18392-1-AP)