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FASTKD2 Polyklonaler Antikörper
FASTKD2 Polyklonal Antikörper für IF, IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (1)
Anwendung
WB, RIP, IP, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 17464-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, HepG2-Zellen, K-562-Zellen |
Erfolgreiche IP | HepG2-Zellen |
Erfolgreiche Detektion in IF | COS7-Zellen, U2OS-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunfluoreszenz (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 4 publications below |
WB | See 11 publications below |
IHC | See 1 publications below |
IF | See 9 publications below |
RIP | See 1 publications below |
Produktinformation
17464-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA FASTKD2 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus, Zebrafisch |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | FASTKD2 fusion protein Ag11314 |
Vollständiger Name | FAST kinase domains 2 |
Berechnetes Molekulargewicht | 710 aa, 81 kDa |
Beobachtetes Molekulargewicht | 55-65 kDa |
GenBank-Zugangsnummer | BC001544 |
Gene symbol | FASTKD2 |
Gene ID (NCBI) | 22868 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)
Protokolle
Produktspezifische Protokolle | |
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WB protocol for FASTKD2 antibody 17464-1-AP | Protokoll herunterladen |
IF protocol for FASTKD2 antibody 17464-1-AP | Protokoll herunterladen |
IP protocol for FASTKD2 antibody 17464-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nat Cell Biol Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics. | ||
Cell Metab A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation. | ||
Nucleic Acids Res FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain. | ||
EMBO Rep A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability. | ||
Cell Rep Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.
| ||
Cell Rep A mitochondria-specific isoform of FASTK is present in mitochondrial RNA granules and regulates gene expression and function. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Erik (Verified Customer) (02-25-2021) | We used the antibody at 1:500 dilution, and it produced two clean bands for the two protein isoforms annotated in UniProt, at the expected height
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