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  • KD/KO Validated

FASTKD2 Polyklonaler Antikörper

FASTKD2 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17464-1-AP

Synonyme

FAST kinase domains 2, FASTKD2, KIAA0971



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HepG2-Zellen, K-562-Zellen
Erfolgreiche IPHepG2-Zellen
Erfolgreiche Detektion in IFCOS7-Zellen, U2OS-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

17464-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA FASTKD2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen FASTKD2 fusion protein Ag11314
Vollständiger Name FAST kinase domains 2
Berechnetes Molekulargewicht 710 aa, 81 kDa
Beobachtetes Molekulargewicht 55-65 kDa
GenBank-ZugangsnummerBC001544
Gene symbol FASTKD2
Gene ID (NCBI) 22868
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)

Protokolle

Produktspezifische Protokolle
WB protocol for FASTKD2 antibody 17464-1-APProtokoll herunterladen
IF protocol for FASTKD2 antibody 17464-1-APProtokoll herunterladen
IP protocol for FASTKD2 antibody 17464-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF,WB

Nat Cell Biol

Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.

Authors - Timo Rey
humanWB

Cell Metab

A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.

Authors - Jason D Arroyo
humanIF

Nucleic Acids Res

FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain.

Authors - Erik Boehm
humanWB

EMBO Rep

A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.

Authors - Hana Antonicka
humanWB, IF, RIP

Cell Rep

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.

Authors - Hana Antonicka
  • KD Validated
IF

Cell Rep

A mitochondria-specific isoform of FASTK is present in mitochondrial RNA granules and regulates gene expression and function.

Authors - Alexis A Jourdain

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Erik (Verified Customer) (02-25-2021)

We used the antibody at 1:500 dilution, and it produced two clean bands for the two protein isoforms annotated in UniProt, at the expected height

  • Applications: Western Blot
  • Cell Tissue Type: PANC1 and MIAPACA2 cell lines
FASTKD2 Antibody Western Blot validation ( dilution) in PANC1 and MIAPACA2 cell lines (Cat no:17464-1-AP)