FASN Monoklonaler Antikörper

FASN Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

CloneNo.

1B6E4

Rezensionen (1)

Kat-Nr. : 66591-1-Ig

Synonyme

FAS, FASN, fatty acid synthase, OA 519, SDR27X1

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Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 66591-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66591-1-Ig (FASN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66591-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66591-1-Ig (FASN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66591-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66591-1-Ig (FASN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66591-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66591-1-Ig (FASN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66591-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66591-1-Ig (FASN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66591-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FASN antibody (66591-1-Ig, Clone: 1B6E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

66591-1-Ig bindet in WB, IP, IHC, IF, ELISA FASN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FASN fusion protein Ag0975
• Vollständiger Name: fatty acid synthase
• Berechnetes Molekulargewicht: 272 kDa
• Beobachtetes Molekulargewicht: 272 kDa
• GenBank-Zugangsnummer: BC007909
• Gene symbol: FASN
• Gene ID (NCBI): 2194
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.

Publikationen

Species	Application	Title
mouse	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
		J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai
human	WB	Theranostics; Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription.; Authors - Gengyi Zou
human	IHC,IP	Am J Cancer Res; Ubiquitin specific peptidase 38 promotes the progression of gastric cancer through upregulation of fatty acid synthase.; Authors - Zhi Zheng
human	WB	Environ Toxicol; SIRT1/mTOR pathway-mediated autophagy dysregulation promotes Pb-induced hepatic lipid accumulation in HepG2 cells; Authors - Yanan Zhao
human	WB	Front Oncol; CRISPR Cas9-mediated ablation of pyruvate carboxylase gene in colon cancer cell line HT-29 inhibits growth and migration, induces apoptosis and increases sensitivity to 5-fluorouracil and glutaminase inhibitor; Authors - Jarunya Ngamkham

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sun (Verified Customer) (01-05-2023)

We detected a band around 200-300kDa region in HEK293T cells using this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T