FANCD2 Polyklonaler Antikörper

FANCD2 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 24006-1-AP

Synonyme

FA D2, FA4, FACD, FAD, FAD2, FANCD, FANCD2, Protein FACD2

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Galerie der Validierungsdaten

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  WB analysis of HL-60 using 24006-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 24006-1-AP (FANCD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 24006-1-AP

  IP Result of anti-FANCD2 (IP:24006-1-AP, 4ug; Detection:24006-1-AP 1:300) with mouse testis tissue lysate 6000ug.

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  IHC staining of human testis using 24006-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 24006-1-AP (FANCD2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 24006-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 24006-1-AP (FANCD2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

24006-1-AP bindet in WB, IP, IHC, IF, ELISA FANCD2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Fanconi anemia, complementation group D2
• Berechnetes Molekulargewicht: 166 kDa
• Beobachtetes Molekulargewicht: 150-155 kDa
• GenBank-Zugangsnummer: NM_033084
• Gene symbol: FANCD2
• Gene ID (NCBI): 2177
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FANCD2, also named as FACD and FACD2, is required for maintenance of chromosomal stability. FANCD2 promotes accurate and efficient pairing of homologs during meiosis. FANCD2 is involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. It may participate in S phase and G2 phase checkpoint activation upon DNA damage. It promotes BRCA2/FANCD1 loading onto damaged chromatin. FANCD2 may also be involved in B-cell immunoglobulin isotype switching. Defects in FANCD2 are a cause of Fanconi anemia (FA) which is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. The antibody recognize both un-phospho and phospho-Ser(330) of FANCD2.

Publikationen

Species	Application	Title
human	WB,IHC	Apoptosis; Crosstalk of ferroptosis regulators and tumor immunity in pancreatic adenocarcinoma: novel perspective to mRNA vaccines and personalized immunotherapy; Authors - Yanlong Shi
human	IHC	BMC Cancer; Comprehensive analysis of the autophagy-dependent ferroptosis-related gene FANCD2 in lung adenocarcinoma.; Authors - Huikai Miao
human	WB	FEBS Open Bio; Augmentation of the therapeutic efficacy of WEE1 kinase inhibitor AZD1775 by inhibiting the YAP-E2F1-DNA damage response pathway axis.; Authors - Yusuke Oku
human	WB,IF	Cell Death Differ; Oxoglutarate dehydrogenase-like inhibits the progression of hepatocellular carcinoma by inducing DNA damage through non-canonical function; Authors - Xiang Jiang
human	WB	Commun Biol; Fanconi anemia associated protein 20 (FAAP20) plays an essential role in homology-directed repair of DNA double-strand breaks; Authors - Anna Palovcak