Phospho-FANCD2 (Ser330) Polyklonaler Antikörper

Phospho-FANCD2 (Ser330) Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19543-1-AP

Synonyme

FA D2, FA4, FACD, FAD, FAD2, FANCD, FANCD2, Phospho-FANCD2 (Ser330), Protein FACD2

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Galerie der Validierungsdaten

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  WB analysis of HL-60 using 19543-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19543-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 19543-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 19543-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 19543-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19543-1-AP bindet in WB, IHC, ELISA Phospho-FANCD2 (Ser330) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Fanconi anemia, complementation group D2
• Berechnetes Molekulargewicht: 166 kDa
• Beobachtetes Molekulargewicht: 150-155 kDa
• GenBank-Zugangsnummer: NM_033084
• Gene symbol: FANCD2
• Gene ID (NCBI): 2177
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FANCD2, also named as FACD and FACD2, is required for maintenance of chromosomal stability. FANCD2 promotes accurate and efficient pairing of homologs during meiosis. FANCD2 is involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. It may participate in S phase and G2 phase checkpoint activation upon DNA damage. It promotes BRCA2/FANCD1 loading onto damaged chromatin. FANCD2 may also be involved in B-cell immunoglobulin isotype switching. Defects in FANCD2 are a cause of Fanconi anemia (FA) which is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. The antibody is to phospho-Ser(330) of FANCD2.