FAM96B Polyklonaler Antikörper

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• KD/KO Validated

FAM96B Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 20108-1-AP

Synonyme

CGI 128, CIA2B, FAM96B, HSPC118, MIP18, Protein FAM96B

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 20108-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Ramos using 20108-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20108-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20108-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20108-1-AP (FAM96B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 20108-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20108-1-AP (FAM96B antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 9 publications below
• CoIP: See 1 publications below

Produktinformation

20108-1-AP bindet in WB, IHC, CoIP, ELISA FAM96B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM96B fusion protein Ag13827
• Vollständiger Name: family with sequence similarity 96, member B
• Berechnetes Molekulargewicht: 163 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC001733
• Gene symbol: FAM96B
• Gene ID (NCBI): 51647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Science; MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.; Authors - Oliver Stehling ; KD Validated
human	WB	Nat Struct Mol Biol; Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators.; Authors - Nima N Naseri
human	WB	Cell Metab; Human CIA2A-FAM96A and CIA2B-FAM96B Integrate Iron Homeostasis and Maturation of Different Subsets of Cytosolic-Nuclear Iron-Sulfur Proteins.; Authors - Stehling Oliver O ; KD Validated
human	WB	Mol Cell; An Oxygen-Dependent Interaction between FBXL5 and the CIA-Targeting Complex Regulates Iron Homeostasis.; Authors - Adarsh K Mayank
human	WB	Cell Rep; The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins.; Authors - Diana C Odermatt
human	WB	J Biol Chem; The Association of the DNA helicase XPD with Transcription Factor IIH is regulated by the Cytosolic Iron-sulfur Cluster Assembly Pathway.; Authors - Ajay A Vashisht