FAM84B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FAM84B Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 18421-1-AP

Synonyme

FAM84B, NSE2, Protein FAM84B, Protein NSE2

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Galerie der Validierungsdaten

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  WB analysis of MDA-MB-453s using 18421-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 18421-1-AP (FAM84B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18421-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18421-1-AP (FAM84B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HUVEC using 18421-1-AP

  IP Result of anti-FAM84B (IP:18421-1-AP, 4ug; Detection:18421-1-AP 1:300) with HUVEC cells lysate 3600ug.

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  IHC staining of human liver cancer using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18421-1-AP (FAM84B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18421-1-AP (FAM84B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human oesophagus cancer using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus cancer using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-453s-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HUVEC-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hodengewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

18421-1-AP bindet in WB, IP, IHC, IF, ELISA FAM84B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM84B fusion protein Ag13243
• Vollständiger Name: family with sequence similarity 84, member B
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC052957
• Gene symbol: FAM84B
• Gene ID (NCBI): 157638
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Nat Genet; Genome-wide profiling of HPV integration in cervical cancer identifies clustered genomic hot spots and a potential microhomology-mediated integration mechanism.; Authors - Zheng Hu
human	WB,IHC	Gigascience; Genomic analyses reveal FAM84B and the NOTCH pathway are associated with the progression of esophageal squamous cell carcinoma.; Authors - Caixia Cheng ; KD Validated
human	IF	Proc Natl Acad Sci U S A; An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking.; Authors - Yan Huang ; KD Validated
human	WB,IHC	Ther Adv Med Oncol; FAM84B promotes prostate tumorigenesis through a network alteration.; Authors - Yanzhi Jiang
human	IHC	Aging (Albany NY); FAM84B, amplified in pancreatic ductal adenocarcinoma, promotes tumorigenesis through the Wnt/β-catenin pathway.; Authors - Xin Zhang
human,mouse	WB,IHC	Oncotarget; Upregulation of FAM84B during prostate cancer progression.; Authors - Nicholas Wong